[12] Site-specific sulfhydryl groups for study of RNA conformation via disulfide cross-linking

Research output: Contribution to journalReview articlepeer-review

Original languageEnglish
Pages (from-to)165-175
Number of pages11
JournalMethods in Enzymology
Volume318
DOIs
Publication statusPublished - 2000

Bibliographical note

Funding Information: We thank J.-P. Behr and G. Zuber who designed and prepared the Sp-Im conjugate, M. Podyminogin who synthesized and first tested the Phen-Im conjugate, and N. Zjdan and D. Konevetz who prepared the Lys-Im and D-Im conjugates. We are indebted to A. Vlassov, I. Kuznetsova, M. Helm, and N. Beloglazova who did the original work with tRNA Asp, tRNA Phe, and the minihelices; we also thank C. Florentz for helpful discussions and constant interest and A. Th6obald-Dietdch for help in tRNA purification. This work was supported by INTAS Grant 96-1418, RFBR Grants 95-03-323691 and 96-15-97732, CNRS, and University Louis Pasteur, Strasbourg.

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