Abstract
The eurybathic isopod species Chelator insignis shows a wide distribution south of Iceland. We analysed 51 specimens from shelf (213-305 m depth), slope (885-891 m and 1380-1390 m depth) and deep-sea habitats (2750 m) south of Iceland with different DNA markers. A fragment of the mitochondrial cytochrome c oxidase subunit I gene (COI) was studied for 47 specimens, 16S was studied for 36 specimens, and a fragment for the 18S rRNA gene could be amplified for 11 specimens. For the COI data, specimens clustered into five distinct lineages each separated by ≥20% uncorrected pairwise distances. Both the mitochondrial 16S and the nuclear 18S sequence data further support this deep divergence, suggesting the presence of overlooked species inside the nominal C. insignis. Populations on the shelf occurring east and west of the Reykjanes Ridge were genetically identical suggesting that this ridge is not a barrier to gene flow. However, populations from different depth ranges differed substantially. Our multi-gene analysis suggests that the newly found species likely have more narrow vertical distribution ranges and highlights a possible role of bathymetry in speciation processes.
| Original language | English |
|---|---|
| Pages (from-to) | 225-242 |
| Number of pages | 18 |
| Journal | Polish Polar Research |
| Volume | 35 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2014 |
Bibliographical note
Funding Information: Acknowledgements. — Captain Michael Schneider and the crew of R/V Meteor and Cap− tain Klaus Ricke and the crew of R/V Poseidon, all scientists on board processing samples and standing up during the nights “to cuddle” samples are thanked for their valuable contribution. We also wish to thank all pickers and sorters on board providing fresh material and the ladies from the Sorting Centre Sandgerđi for their help in isopod determination, especially Sigrún Haraldsdóttir, and the technical assistance of the DZMB Hamburg for the sorting process at home. Karen Jeskulke was our right hand in producing the sequence data. Without her enthusi− asm and all her efforts the quick production of sequences this study would not have been possi− ble. Special thanks go out to Amy Driskell and Karen Osborn at the Smithsonian Institution making our research possible and supporting it in the framework of the Barcoding deep−sea Isopoda project. We also wish to thank the University of Iceland´s Research Centre in Suđurnes for kindly hosting people associated with this project during several workshops. Financial sup− port for S.B. was provided by the German Science Foundation (DFG) under contracts BR 3843/2,3,4,5−1. F.L. was supported by DFG grant MA 3684/2−1.Other keywords
- DNA barcoding
- Desmosomatidae
- Distribution
- Genetic diversity
- Icelandic waters
- Phylogeography