Antimicrobial peptides in the first line defence of human colon mucosa

Maria Tollin; Peter Bergman; Torgny Svenberg; Hans Jörnvall; Gudmundur Hrafn Gudmundsson; Birgitta Agerberth

doi:10.1016/S0196-9781(03)00114-1

Antimicrobial peptides in the first line defence of human colon mucosa

Maria Tollin, Peter Bergman, Torgny Svenberg, Hans Jörnvall, Gudmundur Hrafn Gudmundsson, Birgitta Agerberth

Faculty of Life and Environmental Sciences

University of Iceland

Research output: Contribution to journal › Article › peer-review

Abstract

Antimicrobial peptides and proteins are effector molecules in the protection of epithelial surfaces. We have evaluated the presence of antimicrobial peptides/proteins that can participate in human colonic defence against microbes. A peptide/protein extract of normal human colon mucosa was found to be active against Gram-positive bacteria, Gram-negative bacteria, and fungi. Four polypeptides with antimicrobial activity were isolated from this material and they were identified by N-terminal amino acid sequence analysis as ubiquicidin, histone H2B, eosinophil cationic protein, and phospholipase A₂ (PLA₂). Using immunodetection and mass spectrometry, LL-37, HNP1-3, and HBD-1 were also identified. Combined, these results indicate that the colon mucosa is protected by a complex mixture of polypeptides, able to kill invading microbes and working in synergy as a barrier against bacterial invasion.

Original language	English
Pages (from-to)	523-530
Number of pages	8
Journal	Peptides
Volume	24
Issue number	4
DOIs	https://doi.org/10.1016/S0196-9781(03)00114-1
Publication status	Published - 1 Apr 2003

Bibliographical note

Funding Information: We thank Mona Bajaj Elliot for kindly providing us with HBD-1 antibody and Carina Palmberg and Monica Lindh for technical assistance. This study was supported by grants from the Swedish Research Council, Petrus and Augusta Hedlund’s foundation, Magnus Bergvall’s Foundation, Ruth and Richard Julin’s Foundation and Prof. Nanna Svartz’ Foundation.

Other keywords

Defensins
Histones
Innate immunity
LL-37
Ubiquicidin

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title = "Antimicrobial peptides in the first line defence of human colon mucosa",

abstract = "Antimicrobial peptides and proteins are effector molecules in the protection of epithelial surfaces. We have evaluated the presence of antimicrobial peptides/proteins that can participate in human colonic defence against microbes. A peptide/protein extract of normal human colon mucosa was found to be active against Gram-positive bacteria, Gram-negative bacteria, and fungi. Four polypeptides with antimicrobial activity were isolated from this material and they were identified by N-terminal amino acid sequence analysis as ubiquicidin, histone H2B, eosinophil cationic protein, and phospholipase A2 (PLA2). Using immunodetection and mass spectrometry, LL-37, HNP1-3, and HBD-1 were also identified. Combined, these results indicate that the colon mucosa is protected by a complex mixture of polypeptides, able to kill invading microbes and working in synergy as a barrier against bacterial invasion.",

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author = "Maria Tollin and Peter Bergman and Torgny Svenberg and Hans J{\"o}rnvall and Gudmundsson, \{Gudmundur Hrafn\} and Birgitta Agerberth",

note = "Funding Information: We thank Mona Bajaj Elliot for kindly providing us with HBD-1 antibody and Carina Palmberg and Monica Lindh for technical assistance. This study was supported by grants from the Swedish Research Council, Petrus and Augusta Hedlund{\textquoteright}s foundation, Magnus Bergvall{\textquoteright}s Foundation, Ruth and Richard Julin{\textquoteright}s Foundation and Prof. Nanna Svartz{\textquoteright} Foundation.",

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doi = "10.1016/S0196-9781(03)00114-1",

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AU - Tollin, Maria

AU - Bergman, Peter

AU - Svenberg, Torgny

AU - Jörnvall, Hans

AU - Gudmundsson, Gudmundur Hrafn

AU - Agerberth, Birgitta

N1 - Funding Information: We thank Mona Bajaj Elliot for kindly providing us with HBD-1 antibody and Carina Palmberg and Monica Lindh for technical assistance. This study was supported by grants from the Swedish Research Council, Petrus and Augusta Hedlund’s foundation, Magnus Bergvall’s Foundation, Ruth and Richard Julin’s Foundation and Prof. Nanna Svartz’ Foundation.

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Y1 - 2003/4/1

N2 - Antimicrobial peptides and proteins are effector molecules in the protection of epithelial surfaces. We have evaluated the presence of antimicrobial peptides/proteins that can participate in human colonic defence against microbes. A peptide/protein extract of normal human colon mucosa was found to be active against Gram-positive bacteria, Gram-negative bacteria, and fungi. Four polypeptides with antimicrobial activity were isolated from this material and they were identified by N-terminal amino acid sequence analysis as ubiquicidin, histone H2B, eosinophil cationic protein, and phospholipase A2 (PLA2). Using immunodetection and mass spectrometry, LL-37, HNP1-3, and HBD-1 were also identified. Combined, these results indicate that the colon mucosa is protected by a complex mixture of polypeptides, able to kill invading microbes and working in synergy as a barrier against bacterial invasion.

AB - Antimicrobial peptides and proteins are effector molecules in the protection of epithelial surfaces. We have evaluated the presence of antimicrobial peptides/proteins that can participate in human colonic defence against microbes. A peptide/protein extract of normal human colon mucosa was found to be active against Gram-positive bacteria, Gram-negative bacteria, and fungi. Four polypeptides with antimicrobial activity were isolated from this material and they were identified by N-terminal amino acid sequence analysis as ubiquicidin, histone H2B, eosinophil cationic protein, and phospholipase A2 (PLA2). Using immunodetection and mass spectrometry, LL-37, HNP1-3, and HBD-1 were also identified. Combined, these results indicate that the colon mucosa is protected by a complex mixture of polypeptides, able to kill invading microbes and working in synergy as a barrier against bacterial invasion.

KW - Defensins

KW - Histones

KW - Innate immunity

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KW - Ubiquicidin

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JO - Peptides

JF - Peptides

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ER -

Antimicrobial peptides in the first line defence of human colon mucosa

Abstract

Bibliographical note

Other keywords

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