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Automatic object detection using DBSCAN for counting intoxicated flies in the Florida assay

  • Christian Bodenstein
  • , Markus Götz
  • , Annika Jansen
  • , Henrike Scholz
  • , Morris Riedel

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

In this paper, we propose an instrumentation and computer vision pipeline that allows automatic object detection on images taken from multiple experimental set ups. We demonstrate the approach by autonomously counting intoxicated flies in the FLORIDA assay. The assay measures the effect of ethanol exposure onto the ability of a vinegar fly Drosophila melanogaster to right itself. The analysis consists of a three-step approach. First, obtaining an image of a large set of individual experiments, second, identify areas containing a single experiment, and third, discover the searched objects within the experiment. For the analysis we facilitate well-known computer vision and machine learning algorithms-namely color segmentation, threshold imaging and DBSCAN. The automation of the experiment enables an unprecedented reproducibility and consistency, while significantly decreasing the manual labor.

Original languageEnglish
Title of host publicationProceedings - 2016 15th IEEE International Conference on Machine Learning and Applications, ICMLA 2016
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages746-751
Number of pages6
ISBN (Electronic)9781509061662
DOIs
Publication statusPublished - 31 Jan 2017
Event15th IEEE International Conference on Machine Learning and Applications, ICMLA 2016 - Anaheim, United States
Duration: 18 Dec 201620 Dec 2016

Publication series

NameProceedings - 2016 15th IEEE International Conference on Machine Learning and Applications, ICMLA 2016

Conference

Conference15th IEEE International Conference on Machine Learning and Applications, ICMLA 2016
Country/TerritoryUnited States
CityAnaheim
Period18/12/1620/12/16

Bibliographical note

Publisher Copyright: © 2016 IEEE.

Other keywords

  • Biology
  • Computer vision
  • DBSCAN
  • Flies
  • Florida assay
  • Genetics
  • Image analysis
  • Machine learning

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