Bioavailability of docosahexaenoic acid 22:6(n-3) from enantiopure triacylglycerols and their regioisomeric counterpart in rats

Kaisa M. Linderborg; Amruta Kulkarni; Ai Zhao; Jian Zhang; Heikki Kallio; Johann D. Magnusson; Gudmundur G. Haraldsson; Yumei Zhang; Baoru Yang

doi:10.1016/j.foodchem.2018.12.130

Bioavailability of docosahexaenoic acid 22:6(n-3) from enantiopure triacylglycerols and their regioisomeric counterpart in rats

Kaisa M. Linderborg, Amruta Kulkarni, Ai Zhao, Jian Zhang, Heikki Kallio, Johann D. Magnusson, Gudmundur G. Haraldsson, Yumei Zhang, Baoru Yang

Faculty of Physical Sciences

University of Iceland

Research output: Contribution to journal › Article › peer-review

Abstract

Lack of synthetic enantiospecific triacylglycerols (TAGs) has hindered our understanding of the impact of TAG structure on the absorption and metabolic fate of fatty acids (FAs). In a five-day feeding trial with mildly (n-3) deficient rats, the bioavailability of docosahexaenoic acid [22:6(n-3), DHA] and stearic acid (18:0) from the two different enantiomers of TAG: sn-22:6(n-3)-18:0-18:0 and sn-18:0-18:0-22:6(n-3), and their regioisomeric TAG: sn-18:0-22:6(n-3)-18:0 was compared. Less secretion of fecal DHA was detected from the sn-2 position compared with the sn-1 and sn-3 positions, but no difference was found in DHA content of the fasting plasma or in the weight of the body or organs. 18:0 was lost to feces mainly as cleaved from the primary positions but also as glycerol-bound. The 5-day intervention in rats was long enough to modify the fatty acid profile of plasma phospholipids.

Original language	English
Pages (from-to)	381-389
Number of pages	9
Journal	Food Chemistry
Volume	283
DOIs	https://doi.org/10.1016/j.foodchem.2018.12.130
Publication status	Published - 15 Jun 2019

Bibliographical note

Funding Information: The authors sincerely thank Mathilda Lintunen, Jasmin Raita and Oluwaseun Raphael Samson for technical assistance in the lipid analysis. The research was funded by the Academy of Finland (Decision No. 310982 ), the Raisio plc Research Foundation , Finland, the Finnish Food Research Foundation , and the National Natural Science Foundation of China (Decision No. 81602845 ). Publisher Copyright: © 2019 Elsevier Ltd

Other keywords

(n-3) PUFAs
Absorption
Docosahexaenoic acid
Enantiospecificity
Plasma and fecal lipids
Regiospecificity
Stearic acid
Structured lipids

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10.1016/j.foodchem.2018.12.130

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@article{a09911a15a884cb7881a0b068831fde2,

title = "Bioavailability of docosahexaenoic acid 22:6(n-3) from enantiopure triacylglycerols and their regioisomeric counterpart in rats",

abstract = "Lack of synthetic enantiospecific triacylglycerols (TAGs) has hindered our understanding of the impact of TAG structure on the absorption and metabolic fate of fatty acids (FAs). In a five-day feeding trial with mildly (n-3) deficient rats, the bioavailability of docosahexaenoic acid [22:6(n-3), DHA] and stearic acid (18:0) from the two different enantiomers of TAG: sn-22:6(n-3)-18:0-18:0 and sn-18:0-18:0-22:6(n-3), and their regioisomeric TAG: sn-18:0-22:6(n-3)-18:0 was compared. Less secretion of fecal DHA was detected from the sn-2 position compared with the sn-1 and sn-3 positions, but no difference was found in DHA content of the fasting plasma or in the weight of the body or organs. 18:0 was lost to feces mainly as cleaved from the primary positions but also as glycerol-bound. The 5-day intervention in rats was long enough to modify the fatty acid profile of plasma phospholipids.",

keywords = "(n-3) PUFAs, Absorption, Docosahexaenoic acid, Enantiospecificity, Plasma and fecal lipids, Regiospecificity, Stearic acid, Structured lipids",

author = "Linderborg, \{Kaisa M.\} and Amruta Kulkarni and Ai Zhao and Jian Zhang and Heikki Kallio and Magnusson, \{Johann D.\} and Haraldsson, \{Gudmundur G.\} and Yumei Zhang and Baoru Yang",

note = "Funding Information: The authors sincerely thank Mathilda Lintunen, Jasmin Raita and Oluwaseun Raphael Samson for technical assistance in the lipid analysis. The research was funded by the Academy of Finland (Decision No. 310982 ), the Raisio plc Research Foundation , Finland, the Finnish Food Research Foundation , and the National Natural Science Foundation of China (Decision No. 81602845 ). Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd",

year = "2019",

month = jun,

day = "15",

doi = "10.1016/j.foodchem.2018.12.130",

language = "English",

volume = "283",

pages = "381--389",

journal = "Food Chemistry",

issn = "0308-8146",

publisher = "Elsevier Ltd.",

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T1 - Bioavailability of docosahexaenoic acid 22:6(n-3) from enantiopure triacylglycerols and their regioisomeric counterpart in rats

AU - Linderborg, Kaisa M.

AU - Kulkarni, Amruta

AU - Zhao, Ai

AU - Zhang, Jian

AU - Kallio, Heikki

AU - Magnusson, Johann D.

AU - Haraldsson, Gudmundur G.

AU - Zhang, Yumei

AU - Yang, Baoru

N1 - Funding Information: The authors sincerely thank Mathilda Lintunen, Jasmin Raita and Oluwaseun Raphael Samson for technical assistance in the lipid analysis. The research was funded by the Academy of Finland (Decision No. 310982 ), the Raisio plc Research Foundation , Finland, the Finnish Food Research Foundation , and the National Natural Science Foundation of China (Decision No. 81602845 ). Publisher Copyright: © 2019 Elsevier Ltd

PY - 2019/6/15

Y1 - 2019/6/15

N2 - Lack of synthetic enantiospecific triacylglycerols (TAGs) has hindered our understanding of the impact of TAG structure on the absorption and metabolic fate of fatty acids (FAs). In a five-day feeding trial with mildly (n-3) deficient rats, the bioavailability of docosahexaenoic acid [22:6(n-3), DHA] and stearic acid (18:0) from the two different enantiomers of TAG: sn-22:6(n-3)-18:0-18:0 and sn-18:0-18:0-22:6(n-3), and their regioisomeric TAG: sn-18:0-22:6(n-3)-18:0 was compared. Less secretion of fecal DHA was detected from the sn-2 position compared with the sn-1 and sn-3 positions, but no difference was found in DHA content of the fasting plasma or in the weight of the body or organs. 18:0 was lost to feces mainly as cleaved from the primary positions but also as glycerol-bound. The 5-day intervention in rats was long enough to modify the fatty acid profile of plasma phospholipids.

AB - Lack of synthetic enantiospecific triacylglycerols (TAGs) has hindered our understanding of the impact of TAG structure on the absorption and metabolic fate of fatty acids (FAs). In a five-day feeding trial with mildly (n-3) deficient rats, the bioavailability of docosahexaenoic acid [22:6(n-3), DHA] and stearic acid (18:0) from the two different enantiomers of TAG: sn-22:6(n-3)-18:0-18:0 and sn-18:0-18:0-22:6(n-3), and their regioisomeric TAG: sn-18:0-22:6(n-3)-18:0 was compared. Less secretion of fecal DHA was detected from the sn-2 position compared with the sn-1 and sn-3 positions, but no difference was found in DHA content of the fasting plasma or in the weight of the body or organs. 18:0 was lost to feces mainly as cleaved from the primary positions but also as glycerol-bound. The 5-day intervention in rats was long enough to modify the fatty acid profile of plasma phospholipids.

KW - (n-3) PUFAs

KW - Absorption

KW - Docosahexaenoic acid

KW - Enantiospecificity

KW - Plasma and fecal lipids

KW - Regiospecificity

KW - Stearic acid

KW - Structured lipids

UR - https://www.scopus.com/pages/publications/85060327828

U2 - 10.1016/j.foodchem.2018.12.130

DO - 10.1016/j.foodchem.2018.12.130

M3 - Article

C2 - 30722887

SN - 0308-8146

VL - 283

SP - 381

EP - 389

JO - Food Chemistry

JF - Food Chemistry

ER -

Bioavailability of docosahexaenoic acid 22:6(n-3) from enantiopure triacylglycerols and their regioisomeric counterpart in rats

Abstract

Bibliographical note

Other keywords

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