Catabolite repressive effects of 5-thio-D-glucose on Saccharomyces cerevisiae.

V. Egilsson; V. Gudnason; A. Jónasdottir; S. Ingvarsson; V. Andresdottir

doi:10.1099/00221287-132-12-3309

Catabolite repressive effects of 5-thio-D-glucose on Saccharomyces cerevisiae.

V. Egilsson
, V. Gudnason
, A. Jónasdottir
, S. Ingvarsson
, V. Andresdottir

University of Iceland, The Institute for Experimental Pathology University of Iceland, Other Icelandic Research Institutions

Research output: Contribution to journal › Article › peer-review

Abstract

The effect of the glucose analogue 5-thio-D-glucose (5TG) on the yeast Saccharomyces cerevisiae was studied. Derepression of mitochondrial respiratory chain cytochromes, alcohol dehydrogenase (isoenzyme II), NADH dehydrogenase and maltase was inhibited by 0.5-2 mM-5TG. Growth rate was only slightly affected. Ethanol was efficiently produced with 2 mM-5TG in medium initially containing 0.25% glucose. Mutants resistant to the growth inhibitory effects of 5TG on glycerol medium showed resistance to the catabolite repressing effects of glucose. Other mutants, known to be catabolite repression resistant, showed resistance to 5TG. The analogue seems to inhibit derepression of glucose repressible enzymes with greater potency than glucose itself.

Original language	English
Pages (from-to)	3309-3313
Number of pages	5
Journal	Journal of General Microbiology
Volume	132
Issue number	12
DOIs	https://doi.org/10.1099/00221287-132-12-3309
Publication status	Published - Dec 1986

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title = "Catabolite repressive effects of 5-thio-D-glucose on Saccharomyces cerevisiae.",

abstract = "The effect of the glucose analogue 5-thio-D-glucose (5TG) on the yeast Saccharomyces cerevisiae was studied. Derepression of mitochondrial respiratory chain cytochromes, alcohol dehydrogenase (isoenzyme II), NADH dehydrogenase and maltase was inhibited by 0.5-2 mM-5TG. Growth rate was only slightly affected. Ethanol was efficiently produced with 2 mM-5TG in medium initially containing 0.25\% glucose. Mutants resistant to the growth inhibitory effects of 5TG on glycerol medium showed resistance to the catabolite repressing effects of glucose. Other mutants, known to be catabolite repression resistant, showed resistance to 5TG. The analogue seems to inhibit derepression of glucose repressible enzymes with greater potency than glucose itself.",

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AU - Egilsson, V.

AU - Gudnason, V.

AU - Jónasdottir, A.

AU - Ingvarsson, S.

AU - Andresdottir, V.

PY - 1986/12

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N2 - The effect of the glucose analogue 5-thio-D-glucose (5TG) on the yeast Saccharomyces cerevisiae was studied. Derepression of mitochondrial respiratory chain cytochromes, alcohol dehydrogenase (isoenzyme II), NADH dehydrogenase and maltase was inhibited by 0.5-2 mM-5TG. Growth rate was only slightly affected. Ethanol was efficiently produced with 2 mM-5TG in medium initially containing 0.25% glucose. Mutants resistant to the growth inhibitory effects of 5TG on glycerol medium showed resistance to the catabolite repressing effects of glucose. Other mutants, known to be catabolite repression resistant, showed resistance to 5TG. The analogue seems to inhibit derepression of glucose repressible enzymes with greater potency than glucose itself.

AB - The effect of the glucose analogue 5-thio-D-glucose (5TG) on the yeast Saccharomyces cerevisiae was studied. Derepression of mitochondrial respiratory chain cytochromes, alcohol dehydrogenase (isoenzyme II), NADH dehydrogenase and maltase was inhibited by 0.5-2 mM-5TG. Growth rate was only slightly affected. Ethanol was efficiently produced with 2 mM-5TG in medium initially containing 0.25% glucose. Mutants resistant to the growth inhibitory effects of 5TG on glycerol medium showed resistance to the catabolite repressing effects of glucose. Other mutants, known to be catabolite repression resistant, showed resistance to 5TG. The analogue seems to inhibit derepression of glucose repressible enzymes with greater potency than glucose itself.

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JO - Journal of General Microbiology

JF - Journal of General Microbiology

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Catabolite repressive effects of 5-thio-D-glucose on Saccharomyces cerevisiae.

Abstract

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