Chitosan and Chitin Hexamers affect expansion and differentiation of mesenchymal stem cells differently

Ramona Lieder; Finnbogi Thormodsson; C. H. Ng; Jon M. Einarsson; Johannes Gislason; Petur H. Petersen; Olafur E. Sigurjonsson

doi:10.1016/j.ijbiomac.2012.07.005

Chitosan and Chitin Hexamers affect expansion and differentiation of mesenchymal stem cells differently

Ramona Lieder
, Finnbogi Thormodsson
, C. H. Ng
, Jon M. Einarsson
, Johannes Gislason
, Petur H. Petersen
, Olafur E. Sigurjonsson

University of Iceland, Reykjavik University, University of Akureyri, Landspitali

Research output: Contribution to journal › Article › peer-review

Abstract

Chitooligosaccharides are of interest as potential drugs due to their bioactivity and water solubility. We compared the effect of acetylated and deacetylated chitooligomers (Hexamers) on short-term expansion (7 days) and osteogenic differentiation of bone-marrow derived, human mesenchymal stem cells in terms of gene expression, cytokine secretion and quality of osteogenic differentiation. We show that chitooligomers affect hMSC gene expression and cytokine secretion, but not mineralization. The effect of chitooligomers was shown to be dependent on the acetylation degree, with significantly stronger effects when cells are stimulated with chitin-derived Hexamers (N-Acetyl Chitohexaose) than with Chitosan Hexamers (Chitohexaose).

Original language	English
Pages (from-to)	675-680
Number of pages	6
Journal	International Journal of Biological Macromolecules
Volume	51
Issue number	4
DOIs	https://doi.org/10.1016/j.ijbiomac.2012.07.005
Publication status	Published - Nov 2012

Bibliographical note

Funding Information: We like to acknowledge the Technology Development Fund and the Icelandic Research Fund , managed by the Icelandic Centre for Research and the Landspitali University research fund for funding.

Other keywords

Chitin Hexamer
Chitosan Hexamer
Mesenchymal stem cell
Osteogenesis
YKL-40

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10.1016/j.ijbiomac.2012.07.005

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@article{16df10d215104de39d7332bbc0f9983f,

title = "Chitosan and Chitin Hexamers affect expansion and differentiation of mesenchymal stem cells differently",

abstract = "Chitooligosaccharides are of interest as potential drugs due to their bioactivity and water solubility. We compared the effect of acetylated and deacetylated chitooligomers (Hexamers) on short-term expansion (7 days) and osteogenic differentiation of bone-marrow derived, human mesenchymal stem cells in terms of gene expression, cytokine secretion and quality of osteogenic differentiation. We show that chitooligomers affect hMSC gene expression and cytokine secretion, but not mineralization. The effect of chitooligomers was shown to be dependent on the acetylation degree, with significantly stronger effects when cells are stimulated with chitin-derived Hexamers (N-Acetyl Chitohexaose) than with Chitosan Hexamers (Chitohexaose).",

keywords = "Chitin Hexamer, Chitosan Hexamer, Mesenchymal stem cell, Osteogenesis, YKL-40",

author = "Ramona Lieder and Finnbogi Thormodsson and Ng, \{C. H.\} and Einarsson, \{Jon M.\} and Johannes Gislason and Petersen, \{Petur H.\} and Sigurjonsson, \{Olafur E.\}",

note = "Funding Information: We like to acknowledge the Technology Development Fund and the Icelandic Research Fund , managed by the Icelandic Centre for Research and the Landspitali University research fund for funding.",

year = "2012",

month = nov,

doi = "10.1016/j.ijbiomac.2012.07.005",

language = "English",

volume = "51",

pages = "675--680",

journal = "International Journal of Biological Macromolecules",

issn = "0141-8130",

publisher = "Elsevier B.V.",

number = "4",

}

TY - JOUR

T1 - Chitosan and Chitin Hexamers affect expansion and differentiation of mesenchymal stem cells differently

AU - Lieder, Ramona

AU - Thormodsson, Finnbogi

AU - Ng, C. H.

AU - Einarsson, Jon M.

AU - Gislason, Johannes

AU - Petersen, Petur H.

AU - Sigurjonsson, Olafur E.

N1 - Funding Information: We like to acknowledge the Technology Development Fund and the Icelandic Research Fund , managed by the Icelandic Centre for Research and the Landspitali University research fund for funding.

PY - 2012/11

Y1 - 2012/11

N2 - Chitooligosaccharides are of interest as potential drugs due to their bioactivity and water solubility. We compared the effect of acetylated and deacetylated chitooligomers (Hexamers) on short-term expansion (7 days) and osteogenic differentiation of bone-marrow derived, human mesenchymal stem cells in terms of gene expression, cytokine secretion and quality of osteogenic differentiation. We show that chitooligomers affect hMSC gene expression and cytokine secretion, but not mineralization. The effect of chitooligomers was shown to be dependent on the acetylation degree, with significantly stronger effects when cells are stimulated with chitin-derived Hexamers (N-Acetyl Chitohexaose) than with Chitosan Hexamers (Chitohexaose).

AB - Chitooligosaccharides are of interest as potential drugs due to their bioactivity and water solubility. We compared the effect of acetylated and deacetylated chitooligomers (Hexamers) on short-term expansion (7 days) and osteogenic differentiation of bone-marrow derived, human mesenchymal stem cells in terms of gene expression, cytokine secretion and quality of osteogenic differentiation. We show that chitooligomers affect hMSC gene expression and cytokine secretion, but not mineralization. The effect of chitooligomers was shown to be dependent on the acetylation degree, with significantly stronger effects when cells are stimulated with chitin-derived Hexamers (N-Acetyl Chitohexaose) than with Chitosan Hexamers (Chitohexaose).

KW - Chitin Hexamer

KW - Chitosan Hexamer

KW - Mesenchymal stem cell

KW - Osteogenesis

KW - YKL-40

UR - https://www.scopus.com/pages/publications/84865557154

U2 - 10.1016/j.ijbiomac.2012.07.005

DO - 10.1016/j.ijbiomac.2012.07.005

M3 - Article

C2 - 22790025

SN - 0141-8130

VL - 51

SP - 675

EP - 680

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

IS - 4

ER -

Chitosan and Chitin Hexamers affect expansion and differentiation of mesenchymal stem cells differently

Abstract

Bibliographical note

Other keywords

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Cite this