Conformational dynamics of single protein molecules studied by direct mechanical manipulation

Pétur O. Heidarsson; Mohsin M. Naqvi; Punam Sonar; Immanuel Valpapuram; Ciro Cecconi

doi:10.1016/B978-0-12-411636-8.00003-1

Conformational dynamics of single protein molecules studied by direct mechanical manipulation

Pétur O. Heidarsson, Mohsin M. Naqvi, Punam Sonar, Immanuel Valpapuram, Ciro Cecconi

Faculty of Physical Sciences

University of Iceland

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Advances in single-molecule manipulation techniques have recently enabled researchers to study a growing array of biological processes in unprecedented detail. Individual molecules can now be manipulated with subnanometer precision along a simple and well-defined reaction coordinate, the molecular end-to-end distance, and their conformational changes can be monitored in real time with ever-improving time resolution. The behavior of biomolecules under tension continues to unravel at an accelerated pace and often in combination with computational studies that reveal the atomistic details of the process under investigation. In this chapter, we explain the basic principles of force spectroscopy techniques, with a focus on optical tweezers, and describe some of the theoretical models used to analyze and interpret single-molecule manipulation data. We then highlight some recent and exciting results that have emerged from this research field on protein folding and protein-ligand interactions.

Original language	English
Title of host publication	Dynamics of Proteins and Nucleic Acids
Publisher	Academic Press Inc.
Pages	93-133
Number of pages	41
ISBN (Print)	9780124116368
DOIs	https://doi.org/10.1016/B978-0-12-411636-8.00003-1
Publication status	Published - 2013

Publication series

Name	Advances in Protein Chemistry and Structural Biology
Volume	92

Bibliographical note

Funding Information: P. O. H. acknowledges the Carlsberg Foundation for financial support. C. C. gratefully acknowledges financial support from Fondazione Cassa di Risparmio di Modena, the EU through a Marie Curie International Reintegration Grant (No. 44952), the Italian MIUR (Grant No. 17DPXLNBEK), and partial support from Italian MIUR FIRB RBPR05JH2P “ITALNANONET.” Dr. Birthe B. Kragelund is thanked for critical reading of parts of this manuscript.

Other keywords

Atomic force microscopy
Mechanical manipulation
Molecular dynamics simulations
Optical tweezers
Protein folding
Protein-ligand interactions
Single molecule

Access to Document

10.1016/B978-0-12-411636-8.00003-1

Cite this

@inbook{a70a1a888a7743f1951b9a4384a277a5,

title = "Conformational dynamics of single protein molecules studied by direct mechanical manipulation",

abstract = "Advances in single-molecule manipulation techniques have recently enabled researchers to study a growing array of biological processes in unprecedented detail. Individual molecules can now be manipulated with subnanometer precision along a simple and well-defined reaction coordinate, the molecular end-to-end distance, and their conformational changes can be monitored in real time with ever-improving time resolution. The behavior of biomolecules under tension continues to unravel at an accelerated pace and often in combination with computational studies that reveal the atomistic details of the process under investigation. In this chapter, we explain the basic principles of force spectroscopy techniques, with a focus on optical tweezers, and describe some of the theoretical models used to analyze and interpret single-molecule manipulation data. We then highlight some recent and exciting results that have emerged from this research field on protein folding and protein-ligand interactions.",

keywords = "Atomic force microscopy, Mechanical manipulation, Molecular dynamics simulations, Optical tweezers, Protein folding, Protein-ligand interactions, Single molecule",

author = "Heidarsson, \{P{\'e}tur O.\} and Naqvi, \{Mohsin M.\} and Punam Sonar and Immanuel Valpapuram and Ciro Cecconi",

note = "Funding Information: P. O. H. acknowledges the Carlsberg Foundation for financial support. C. C. gratefully acknowledges financial support from Fondazione Cassa di Risparmio di Modena, the EU through a Marie Curie International Reintegration Grant (No. 44952), the Italian MIUR (Grant No. 17DPXLNBEK), and partial support from Italian MIUR FIRB RBPR05JH2P “ITALNANONET.” Dr. Birthe B. Kragelund is thanked for critical reading of parts of this manuscript.",

year = "2013",

doi = "10.1016/B978-0-12-411636-8.00003-1",

language = "English",

isbn = "9780124116368",

series = "Advances in Protein Chemistry and Structural Biology",

publisher = "Academic Press Inc.",

pages = "93--133",

booktitle = "Dynamics of Proteins and Nucleic Acids",

address = "United States",

}

Conformational dynamics of single protein molecules studied by direct mechanical manipulation. / Heidarsson, Pétur O.; Naqvi, Mohsin M.; Sonar, Punam et al.
Dynamics of Proteins and Nucleic Acids. Academic Press Inc., 2013. p. 93-133 (Advances in Protein Chemistry and Structural Biology; Vol. 92).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

TY - CHAP

T1 - Conformational dynamics of single protein molecules studied by direct mechanical manipulation

AU - Heidarsson, Pétur O.

AU - Naqvi, Mohsin M.

AU - Sonar, Punam

AU - Valpapuram, Immanuel

AU - Cecconi, Ciro

N1 - Funding Information: P. O. H. acknowledges the Carlsberg Foundation for financial support. C. C. gratefully acknowledges financial support from Fondazione Cassa di Risparmio di Modena, the EU through a Marie Curie International Reintegration Grant (No. 44952), the Italian MIUR (Grant No. 17DPXLNBEK), and partial support from Italian MIUR FIRB RBPR05JH2P “ITALNANONET.” Dr. Birthe B. Kragelund is thanked for critical reading of parts of this manuscript.

PY - 2013

Y1 - 2013

N2 - Advances in single-molecule manipulation techniques have recently enabled researchers to study a growing array of biological processes in unprecedented detail. Individual molecules can now be manipulated with subnanometer precision along a simple and well-defined reaction coordinate, the molecular end-to-end distance, and their conformational changes can be monitored in real time with ever-improving time resolution. The behavior of biomolecules under tension continues to unravel at an accelerated pace and often in combination with computational studies that reveal the atomistic details of the process under investigation. In this chapter, we explain the basic principles of force spectroscopy techniques, with a focus on optical tweezers, and describe some of the theoretical models used to analyze and interpret single-molecule manipulation data. We then highlight some recent and exciting results that have emerged from this research field on protein folding and protein-ligand interactions.

AB - Advances in single-molecule manipulation techniques have recently enabled researchers to study a growing array of biological processes in unprecedented detail. Individual molecules can now be manipulated with subnanometer precision along a simple and well-defined reaction coordinate, the molecular end-to-end distance, and their conformational changes can be monitored in real time with ever-improving time resolution. The behavior of biomolecules under tension continues to unravel at an accelerated pace and often in combination with computational studies that reveal the atomistic details of the process under investigation. In this chapter, we explain the basic principles of force spectroscopy techniques, with a focus on optical tweezers, and describe some of the theoretical models used to analyze and interpret single-molecule manipulation data. We then highlight some recent and exciting results that have emerged from this research field on protein folding and protein-ligand interactions.

KW - Atomic force microscopy

KW - Mechanical manipulation

KW - Molecular dynamics simulations

KW - Optical tweezers

KW - Protein folding

KW - Protein-ligand interactions

KW - Single molecule

UR - https://www.scopus.com/pages/publications/84896701259

U2 - 10.1016/B978-0-12-411636-8.00003-1

DO - 10.1016/B978-0-12-411636-8.00003-1

M3 - Chapter

C2 - 23954100

SN - 9780124116368

T3 - Advances in Protein Chemistry and Structural Biology

SP - 93

EP - 133

BT - Dynamics of Proteins and Nucleic Acids

PB - Academic Press Inc.

ER -

Conformational dynamics of single protein molecules studied by direct mechanical manipulation

Abstract

Publication series

Bibliographical note

Other keywords

Access to Document

Fingerprint

Cite this