Construction and characterization of an infectious molecular clone of maedi-visna virus that expresses green fluorescent protein

Hólmfrídur Sunna Gudmundsdóttir; Katrín Olafsdóttir; Sigrídur Rut Franzdóttir; Valgerdur Andrésdóttir

doi:10.1016/j.jviromet.2010.04.026

Construction and characterization of an infectious molecular clone of maedi-visna virus that expresses green fluorescent protein

Hólmfrídur Sunna Gudmundsdóttir
, Katrín Olafsdóttir
, Sigrídur Rut Franzdóttir
, Valgerdur Andrésdóttir

Faculty of Life and Environmental Sciences

University of Iceland

Research output: Contribution to journal › Article › peer-review

Abstract

The construction of a molecular clone of maedi-visna virus (MVV) expressing the enhanced green fluorescent protein (EGFP) is described. The egfp gene was inserted into the gene for dUTPase since it has been shown that dUTPase is dispensable for MVV replication both in vitro and in vivo. MVV-egfp is infectious and EGFP expression is stable over at least six passages. This fluorescent virus will be a useful tool for monitoring MVV infections.

Original language	English
Pages (from-to)	98-102
Number of pages	5
Journal	Journal of Virological Methods
Volume	168
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jviromet.2010.04.026
Publication status	Published - Sept 2010

Bibliographical note

Funding Information: This work was supported by grants from The Icelandic Research Fund and the University of Iceland Research Fund .

Other keywords

EGFP
Lentivirus
Maedi-visna virus (MVV)

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10.1016/j.jviromet.2010.04.026

Cite this

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title = "Construction and characterization of an infectious molecular clone of maedi-visna virus that expresses green fluorescent protein",

abstract = "The construction of a molecular clone of maedi-visna virus (MVV) expressing the enhanced green fluorescent protein (EGFP) is described. The egfp gene was inserted into the gene for dUTPase since it has been shown that dUTPase is dispensable for MVV replication both in vitro and in vivo. MVV-egfp is infectious and EGFP expression is stable over at least six passages. This fluorescent virus will be a useful tool for monitoring MVV infections.",

keywords = "EGFP, Lentivirus, Maedi-visna virus (MVV)",

author = "Gudmundsd{\'o}ttir, \{H{\'o}lmfr{\'i}dur Sunna\} and Katr{\'i}n Olafsd{\'o}ttir and Franzd{\'o}ttir, \{Sigr{\'i}dur Rut\} and Valgerdur Andr{\'e}sd{\'o}ttir",

note = "Funding Information: This work was supported by grants from The Icelandic Research Fund and the University of Iceland Research Fund .",

year = "2010",

month = sep,

doi = "10.1016/j.jviromet.2010.04.026",

language = "English",

volume = "168",

pages = "98--102",

journal = "Journal of Virological Methods",

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publisher = "Elsevier B.V.",

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T1 - Construction and characterization of an infectious molecular clone of maedi-visna virus that expresses green fluorescent protein

AU - Gudmundsdóttir, Hólmfrídur Sunna

AU - Olafsdóttir, Katrín

AU - Franzdóttir, Sigrídur Rut

AU - Andrésdóttir, Valgerdur

N1 - Funding Information: This work was supported by grants from The Icelandic Research Fund and the University of Iceland Research Fund .

PY - 2010/9

Y1 - 2010/9

N2 - The construction of a molecular clone of maedi-visna virus (MVV) expressing the enhanced green fluorescent protein (EGFP) is described. The egfp gene was inserted into the gene for dUTPase since it has been shown that dUTPase is dispensable for MVV replication both in vitro and in vivo. MVV-egfp is infectious and EGFP expression is stable over at least six passages. This fluorescent virus will be a useful tool for monitoring MVV infections.

AB - The construction of a molecular clone of maedi-visna virus (MVV) expressing the enhanced green fluorescent protein (EGFP) is described. The egfp gene was inserted into the gene for dUTPase since it has been shown that dUTPase is dispensable for MVV replication both in vitro and in vivo. MVV-egfp is infectious and EGFP expression is stable over at least six passages. This fluorescent virus will be a useful tool for monitoring MVV infections.

KW - EGFP

KW - Lentivirus

KW - Maedi-visna virus (MVV)

UR - https://www.scopus.com/pages/publications/77955094711

U2 - 10.1016/j.jviromet.2010.04.026

DO - 10.1016/j.jviromet.2010.04.026

M3 - Article

C2 - 20447418

SN - 0166-0934

VL - 168

SP - 98

EP - 102

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 1-2

ER -

Construction and characterization of an infectious molecular clone of maedi-visna virus that expresses green fluorescent protein

Abstract

Bibliographical note

Other keywords

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