Effect of mechanical agitation on hybridoma cell growth

Gyun Min Lee; Thomas K. Huard; Mark S. Kaminski; Bernhard O. Palsson

doi:10.1007/BF01024713

Effect of mechanical agitation on hybridoma cell growth

Gyun Min Lee, Thomas K. Huard, Mark S. Kaminski, Bernhard O. Palsson

University of Iceland

University of Iceland

Research output: Contribution to journal › Article › peer-review

Abstract

Hybridoma cells (S3H5/γ2bA2) were grown in spinner flasks at different agitation speeds. It was found that cells in stationary and decline phases of growth were sensitive to shear force caused by agitation but cells in growth phase seemed less sensitive to the shear forces introduced. The death rate was found to be. 0.007 hr^-1 in T flasks but 0.018 hr^-1 and 0.028 hr^-1 at 100 and 200 rpm, respectively, while the growth rate was about 0.05 hr^-1 for all cases.

Original language	English
Pages (from-to)	625-628
Number of pages	4
Journal	Biotechnology Letters
Volume	10
Issue number	9
DOIs	https://doi.org/10.1007/BF01024713
Publication status	Published - Sept 1988

Access to Document

10.1007/BF01024713

Cite this

@article{bd481a4272d349d58b58676b68755a3a,

title = "Effect of mechanical agitation on hybridoma cell growth",

abstract = "Hybridoma cells (S3H5/γ2bA2) were grown in spinner flasks at different agitation speeds. It was found that cells in stationary and decline phases of growth were sensitive to shear force caused by agitation but cells in growth phase seemed less sensitive to the shear forces introduced. The death rate was found to be. 0.007 hr-1 in T flasks but 0.018 hr-1 and 0.028 hr-1 at 100 and 200 rpm, respectively, while the growth rate was about 0.05 hr-1 for all cases.",

author = "Lee, \{Gyun Min\} and Huard, \{Thomas K.\} and Kaminski, \{Mark S.\} and Palsson, \{Bernhard O.\}",

year = "1988",

month = sep,

doi = "10.1007/BF01024713",

language = "English",

volume = "10",

pages = "625--628",

journal = "Biotechnology Letters",

issn = "0141-5492",

publisher = "Springer Science and Business Media B.V.",

number = "9",

}

TY - JOUR

T1 - Effect of mechanical agitation on hybridoma cell growth

AU - Lee, Gyun Min

AU - Huard, Thomas K.

AU - Kaminski, Mark S.

AU - Palsson, Bernhard O.

PY - 1988/9

Y1 - 1988/9

N2 - Hybridoma cells (S3H5/γ2bA2) were grown in spinner flasks at different agitation speeds. It was found that cells in stationary and decline phases of growth were sensitive to shear force caused by agitation but cells in growth phase seemed less sensitive to the shear forces introduced. The death rate was found to be. 0.007 hr-1 in T flasks but 0.018 hr-1 and 0.028 hr-1 at 100 and 200 rpm, respectively, while the growth rate was about 0.05 hr-1 for all cases.

AB - Hybridoma cells (S3H5/γ2bA2) were grown in spinner flasks at different agitation speeds. It was found that cells in stationary and decline phases of growth were sensitive to shear force caused by agitation but cells in growth phase seemed less sensitive to the shear forces introduced. The death rate was found to be. 0.007 hr-1 in T flasks but 0.018 hr-1 and 0.028 hr-1 at 100 and 200 rpm, respectively, while the growth rate was about 0.05 hr-1 for all cases.

UR - https://www.scopus.com/pages/publications/0023788898

U2 - 10.1007/BF01024713

DO - 10.1007/BF01024713

M3 - Article

SN - 0141-5492

VL - 10

SP - 625

EP - 628

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 9

ER -

Effect of mechanical agitation on hybridoma cell growth

Abstract

Access to Document

Fingerprint

Cite this