TY - JOUR
T1 - Identification of a potent antibacterial factor isolated from bronchoalveolar lavage fluid
T2 - Guanidine, N-[3-[(aminoiminomethyl)amino]propyl]-N-dodecyl-, a potential source of error in the analysis of antibacterial agents
AU - Abraham-Nordling, Mirna
AU - Guðmundsson, Guðmundur H
AU - Grunewald, Johan
AU - Agerberth, Birgitta
AU - Griffiths, William J.
PY - 2003
Y1 - 2003
N2 - The widespread use of antibiotics in modern society has encouraged the search for new antibacterial compounds. In this laboratory investigations are being made to identify and characterise novel antibacterial peptides. With this in mind, the antibacterial properties of human bronchoalveolar lavage (BAL) fluid from sarcoidosis patients is being investigated. In this communication we report on the identification and characterisation of a highly active non-peptide antibacterial compound isolated from BAL fluid. The structure of this active compound was elucidated by high-resolution accurate mass and tandem mass spectrometry to be guanidine, N-[3-[(aminoiminomethyl)amino]propyl]-N-dodecyl-. This compound does not appear to be endogenous, and its presence in BAL fluid extracts presents a potential source of error in analysis of antibacterial agents. The biological effects of guanidine, N-[3-[(aminoiminomethyl)amino]propyl]-N-dodecyl- have not previously been described in the literature.
AB - The widespread use of antibiotics in modern society has encouraged the search for new antibacterial compounds. In this laboratory investigations are being made to identify and characterise novel antibacterial peptides. With this in mind, the antibacterial properties of human bronchoalveolar lavage (BAL) fluid from sarcoidosis patients is being investigated. In this communication we report on the identification and characterisation of a highly active non-peptide antibacterial compound isolated from BAL fluid. The structure of this active compound was elucidated by high-resolution accurate mass and tandem mass spectrometry to be guanidine, N-[3-[(aminoiminomethyl)amino]propyl]-N-dodecyl-. This compound does not appear to be endogenous, and its presence in BAL fluid extracts presents a potential source of error in analysis of antibacterial agents. The biological effects of guanidine, N-[3-[(aminoiminomethyl)amino]propyl]-N-dodecyl- have not previously been described in the literature.
UR - https://www.scopus.com/pages/publications/12244296134
U2 - 10.1002/rcm.891
DO - 10.1002/rcm.891
M3 - Article
C2 - 12539181
SN - 0951-4198
VL - 17
SP - 183
EP - 191
JO - Rapid Communications in Mass Spectrometry
JF - Rapid Communications in Mass Spectrometry
IS - 3
ER -