Abstract
Hybridoma cells (S3H5/γ2bA2) were cultivated in spinner flasks with 1% serum media and serum‐free media. Monoclonal antibody productivity was maintained in 1% serum media. However, cells in serum‐free media showed a decrease in antibody productivity, and it completely disappeared in IMDM‐based low protein medium. This loss of antibody productivity was not observed when the cells were immobilized in alginate beads. In fact, immobilization enhanced the specific MAb productivity.
| Original language | English |
|---|---|
| Pages (from-to) | 1049-1055 |
| Number of pages | 7 |
| Journal | Biotechnology and Bioengineering |
| Volume | 36 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 5 Dec 1990 |
Fingerprint
Dive into the research topics of 'Immobilization can improve the stability of hybridoma antibody productivity in serum‐free media'. Together they form a unique fingerprint.Cite this
- APA
- Author
- BIBTEX
- Harvard
- Standard
- RIS
- Vancouver