Isolation and characterization of lambda transducing phages for the E. coli genes ksgA and pdxA

Ólafur Sigmar Andrésson; Julian E. Davies

doi:10.1007/BF00268464

Isolation and characterization of lambda transducing phages for the E. coli genes ksgA and pdxA

Ólafur Sigmar Andrésson, Julian E. Davies

Faculty of Life and Environmental Sciences

University of Iceland

Research output: Contribution to journal › Article › peer-review

Abstract

Lambda phages carrying the Escherichia coli genes ksgA and pdxA were isolated from secondary site lysogens in araB. 1) The phage genomes were characterized by genetic complementation tests, restriction endonuclease digestion and electron microscopy. 2) A 6.3 kilobasepair (kb) EcoRI restriction fragment carrying both ksgA and pdxA was cloned in a lambda vector; this fragment has proven useful in further characterization of the ksgA gene (Andrésson and Davies, 1980a, b). The ksgA and pdxA genes are about 14 and 12-13 kb, respectively, counterclockwise of the arabinose operon and 1.5 and 2.5-3.5 kb clockwise of folA.

Original language	English
Pages (from-to)	201-209
Number of pages	9
Journal	MGG Molecular & General Genetics
Volume	179
Issue number	1
DOIs	https://doi.org/10.1007/BF00268464
Publication status	Published - Aug 1980

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title = "Isolation and characterization of lambda transducing phages for the E. coli genes ksgA and pdxA",

abstract = "Lambda phages carrying the Escherichia coli genes ksgA and pdxA were isolated from secondary site lysogens in araB. 1) The phage genomes were characterized by genetic complementation tests, restriction endonuclease digestion and electron microscopy. 2) A 6.3 kilobasepair (kb) EcoRI restriction fragment carrying both ksgA and pdxA was cloned in a lambda vector; this fragment has proven useful in further characterization of the ksgA gene (Andr{\'e}sson and Davies, 1980a, b). The ksgA and pdxA genes are about 14 and 12-13 kb, respectively, counterclockwise of the arabinose operon and 1.5 and 2.5-3.5 kb clockwise of folA.",

author = "Andr{\'e}sson, \{{\'O}lafur Sigmar\} and Davies, \{Julian E.\}",

note = "Copyright {\textcopyright} 1969, Springer-Verlag",

year = "1980",

month = aug,

doi = "10.1007/BF00268464",

language = "English",

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journal = "MGG Molecular \& General Genetics",

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AU - Andrésson, Ólafur Sigmar

AU - Davies, Julian E.

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N2 - Lambda phages carrying the Escherichia coli genes ksgA and pdxA were isolated from secondary site lysogens in araB. 1) The phage genomes were characterized by genetic complementation tests, restriction endonuclease digestion and electron microscopy. 2) A 6.3 kilobasepair (kb) EcoRI restriction fragment carrying both ksgA and pdxA was cloned in a lambda vector; this fragment has proven useful in further characterization of the ksgA gene (Andrésson and Davies, 1980a, b). The ksgA and pdxA genes are about 14 and 12-13 kb, respectively, counterclockwise of the arabinose operon and 1.5 and 2.5-3.5 kb clockwise of folA.

AB - Lambda phages carrying the Escherichia coli genes ksgA and pdxA were isolated from secondary site lysogens in araB. 1) The phage genomes were characterized by genetic complementation tests, restriction endonuclease digestion and electron microscopy. 2) A 6.3 kilobasepair (kb) EcoRI restriction fragment carrying both ksgA and pdxA was cloned in a lambda vector; this fragment has proven useful in further characterization of the ksgA gene (Andrésson and Davies, 1980a, b). The ksgA and pdxA genes are about 14 and 12-13 kb, respectively, counterclockwise of the arabinose operon and 1.5 and 2.5-3.5 kb clockwise of folA.

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JF - MGG Molecular & General Genetics

IS - 1

ER -

Isolation and characterization of lambda transducing phages for the E. coli genes ksgA and pdxA

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