TY - JOUR
T1 - Isolation and characterization of Thermus thermophilus Gy1211 from a deep-sea hydrothermal vent
AU - Marteinsson, Viggó Thór
AU - Birrien, Jean Louis
AU - Raguénès, Gérard
AU - Da Costa, Milton S.
AU - Prieur, Daniel
N1 - Funding Information: Acknowledgments We thank Anne-Marie Alayse, chief scientist of the cruise “Guaynaut”, for inviting one of us to participate in that cruise. Thanks are also due to Anne Donval and Gérard Sinquin for the electron microscopy Viggó Thór Marteinsson was supported by a French-Icelandic fellowship (Programme Franco-Islandais). This work was supported by GDR 1006 CNRS/Ifremer, the CPER 94–95 (Contrat de Plan Etat-Région), the European Union (Feder, objectif 5b), and Conseil Général du Finistèr.
PY - 1999/11
Y1 - 1999/11
N2 - We examined a single, non-spore-forming, aerobic, thermophilic strain that was isolated from a deep-sea hydrothermal vent in the Guaymas Basin at a depth of 2000 m and initially placed in a phenetic group with Thermus scotoductus (X-1). We identified this deep-sea isolate as a new strain belonging to Thermus thermophilus using several parameters. DNA-DNA hybridization under stringent conditions showed 74% similarity between the deep-sea isolate and T. thermophilus HB-8(T) (T = type strain). Phenotypic characteristics, such as the utilization of carbon sources, hydrolysis of different compounds, and antibiotic sensitivity were identical in the two strains. The polar lipids composition showed that strain Gy1211 belonged to the genus Thermus. The fatty acids composition indicated that this strain was related to the marine T. thermophilus strain isolated from the Azores. The new isolate T. thermophilus strain Gy1211 grew optimally at 75°C, pH 8.0, and 2% NaCl. A hydrostatic pressure of 20MPa, similar to the in situ hydrostatic pressure of the deep-sea vent from which the strain was isolated, had no effect on growth. Strain HB-8(T), however, showed slower growth under these conditions.
AB - We examined a single, non-spore-forming, aerobic, thermophilic strain that was isolated from a deep-sea hydrothermal vent in the Guaymas Basin at a depth of 2000 m and initially placed in a phenetic group with Thermus scotoductus (X-1). We identified this deep-sea isolate as a new strain belonging to Thermus thermophilus using several parameters. DNA-DNA hybridization under stringent conditions showed 74% similarity between the deep-sea isolate and T. thermophilus HB-8(T) (T = type strain). Phenotypic characteristics, such as the utilization of carbon sources, hydrolysis of different compounds, and antibiotic sensitivity were identical in the two strains. The polar lipids composition showed that strain Gy1211 belonged to the genus Thermus. The fatty acids composition indicated that this strain was related to the marine T. thermophilus strain isolated from the Azores. The new isolate T. thermophilus strain Gy1211 grew optimally at 75°C, pH 8.0, and 2% NaCl. A hydrostatic pressure of 20MPa, similar to the in situ hydrostatic pressure of the deep-sea vent from which the strain was isolated, had no effect on growth. Strain HB-8(T), however, showed slower growth under these conditions.
KW - Hydrothermal vents
KW - Marine
KW - Taxonomy
KW - Thermophiles
KW - Thermus thermophilus
UR - https://www.scopus.com/pages/publications/0033227459
U2 - 10.1007/s007920050123
DO - 10.1007/s007920050123
M3 - Article
C2 - 10591014
SN - 1431-0651
VL - 3
SP - 247
EP - 251
JO - Extremophiles
JF - Extremophiles
IS - 4
ER -