TY - JOUR
T1 - Phylogeny, processing and expression of the rat cathelicidin rCRAMP
T2 - A model for innate antimicrobial peptides
AU - Térmen, S.
AU - Tollin, M.
AU - Olsson, B.
AU - Svenberg, T.
AU - Agerberth, B.
AU - Gudmundsson, G. H.
PY - 2003/3/1
Y1 - 2003/3/1
N2 - A database search identified a rat cDNA clone which phylogenetic analysis revealed to encode a cathelicidin most similar to mouse cathelicidin CRAMP. The analysis also showed that the evolutionary pattern of the cathelicidin family is lineage specific. The rat cathelicidin is called rCRAMP. Its peptide was isolated from granulocytes, and determined to be 43 amino acids long by mass spectrometry and N-terminal sequencing. Synthetic rCRAMP had antimicrobial activity. The expression of rCRAMP was investigated by reverse-transcriptase polymerase chain reaction followed by Southern hybridization and by Western blot analysis. rCRAMP was identified in granulocytes, thymus, testis, lung, mouth mucosa, tongue, oesophagus, colon, caecum and small intestine, a distribution similar to cathelicidins of mouse and human. The rat is a small laboratory animal with additional disease models available compared to the mouse. Our results open up the possibility to use the rat as a model system to study responses connected to cathelicidin expression in health and disease.
AB - A database search identified a rat cDNA clone which phylogenetic analysis revealed to encode a cathelicidin most similar to mouse cathelicidin CRAMP. The analysis also showed that the evolutionary pattern of the cathelicidin family is lineage specific. The rat cathelicidin is called rCRAMP. Its peptide was isolated from granulocytes, and determined to be 43 amino acids long by mass spectrometry and N-terminal sequencing. Synthetic rCRAMP had antimicrobial activity. The expression of rCRAMP was investigated by reverse-transcriptase polymerase chain reaction followed by Southern hybridization and by Western blot analysis. rCRAMP was identified in granulocytes, thymus, testis, lung, mouth mucosa, tongue, oesophagus, colon, caecum and small intestine, a distribution similar to cathelicidins of mouse and human. The rat is a small laboratory animal with additional disease models available compared to the mouse. Our results open up the possibility to use the rat as a model system to study responses connected to cathelicidin expression in health and disease.
KW - Antimicrobial peptide
KW - Cathelicidin
KW - Expression
KW - Phylogeny
KW - Processing
KW - Rat
KW - rCRAMP
UR - https://www.scopus.com/pages/publications/0344080624
U2 - 10.1007/s000180300045
DO - 10.1007/s000180300045
M3 - Article
C2 - 12737313
SN - 1420-682X
VL - 60
SP - 536
EP - 549
JO - Cellular and Molecular Life Sciences
JF - Cellular and Molecular Life Sciences
IS - 3
ER -