Quartz Crystal Microbalance Bioaffinity Sensor for Biotin

Már Masson; Kyusik Yun; Tetsuya Haruyama; Eiry Kobatake; Masuo Aizawa

doi:10.1021/ac00109a047

Quartz Crystal Microbalance Bioaffinity Sensor for Biotin

Már Masson, Kyusik Yun, Tetsuya Haruyama, Eiry Kobatake, Masuo Aizawa

Faculty of Pharmaceutical Sciences

University of Iceland

Research output: Contribution to journal › Article › peer-review

Abstract

A quartz crystal microbalance (QCM) bioaffinity sensor for biotin, based on the bioaffinity complex between avidin and immobilized desthiobiotin, was established. BSA-desthiobiotin conjugate was adsorbed directly onto the gold layer surface of a quartz crystal. The crystal was placed in a QCM flow cell. Avidin formed a metastable complex with the BSA-desthiobiotin conjugate on the crystal surface. When a sample solution containing biotin was injected, the avidin was released from the surface to form a more stable complex with biotin in solution. The biotin concentration corresponded to the weight change on the crystal surface that occurred when avidin desorbed. Measurements were done in the stop-flow mode with a measurement range between 0.05 and 100 μg/mL and in the continuous-flow mode with a measurement range between 0.2 and 10000 μg/mL. The sensor was regenerated without any loss of activity by readsorbing avidin.

Original language	English
Pages (from-to)	2212-2215
Number of pages	4
Journal	Analytical Chemistry
Volume	67
Issue number	13
DOIs	https://doi.org/10.1021/ac00109a047
Publication status	Published - 1 Jul 1995

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title = "Quartz Crystal Microbalance Bioaffinity Sensor for Biotin",

abstract = "A quartz crystal microbalance (QCM) bioaffinity sensor for biotin, based on the bioaffinity complex between avidin and immobilized desthiobiotin, was established. BSA-desthiobiotin conjugate was adsorbed directly onto the gold layer surface of a quartz crystal. The crystal was placed in a QCM flow cell. Avidin formed a metastable complex with the BSA-desthiobiotin conjugate on the crystal surface. When a sample solution containing biotin was injected, the avidin was released from the surface to form a more stable complex with biotin in solution. The biotin concentration corresponded to the weight change on the crystal surface that occurred when avidin desorbed. Measurements were done in the stop-flow mode with a measurement range between 0.05 and 100 μg/mL and in the continuous-flow mode with a measurement range between 0.2 and 10000 μg/mL. The sensor was regenerated without any loss of activity by readsorbing avidin.",

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T1 - Quartz Crystal Microbalance Bioaffinity Sensor for Biotin

AU - Masson, Már

AU - Yun, Kyusik

AU - Haruyama, Tetsuya

AU - Kobatake, Eiry

AU - Aizawa, Masuo

PY - 1995/7/1

Y1 - 1995/7/1

N2 - A quartz crystal microbalance (QCM) bioaffinity sensor for biotin, based on the bioaffinity complex between avidin and immobilized desthiobiotin, was established. BSA-desthiobiotin conjugate was adsorbed directly onto the gold layer surface of a quartz crystal. The crystal was placed in a QCM flow cell. Avidin formed a metastable complex with the BSA-desthiobiotin conjugate on the crystal surface. When a sample solution containing biotin was injected, the avidin was released from the surface to form a more stable complex with biotin in solution. The biotin concentration corresponded to the weight change on the crystal surface that occurred when avidin desorbed. Measurements were done in the stop-flow mode with a measurement range between 0.05 and 100 μg/mL and in the continuous-flow mode with a measurement range between 0.2 and 10000 μg/mL. The sensor was regenerated without any loss of activity by readsorbing avidin.

AB - A quartz crystal microbalance (QCM) bioaffinity sensor for biotin, based on the bioaffinity complex between avidin and immobilized desthiobiotin, was established. BSA-desthiobiotin conjugate was adsorbed directly onto the gold layer surface of a quartz crystal. The crystal was placed in a QCM flow cell. Avidin formed a metastable complex with the BSA-desthiobiotin conjugate on the crystal surface. When a sample solution containing biotin was injected, the avidin was released from the surface to form a more stable complex with biotin in solution. The biotin concentration corresponded to the weight change on the crystal surface that occurred when avidin desorbed. Measurements were done in the stop-flow mode with a measurement range between 0.05 and 100 μg/mL and in the continuous-flow mode with a measurement range between 0.2 and 10000 μg/mL. The sensor was regenerated without any loss of activity by readsorbing avidin.

UR - https://www.scopus.com/pages/publications/0000171877

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JO - Analytical Chemistry

JF - Analytical Chemistry

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ER -

Quartz Crystal Microbalance Bioaffinity Sensor for Biotin

Abstract

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