RAD sequencing of common whelk, Buccinum undatum, reveals fine-scale population structuring in Europe and cryptic speciation within the North Atlantic

Jake Goodall; Kristen Marie Westfall; Hildur Magnúsdóttir; Snæbjörn Pálsson; Erla Björk Örnólfsdóttir; Zophonías O. Jónsson

doi:10.1002/ece3.7219

RAD sequencing of common whelk, Buccinum undatum, reveals fine-scale population structuring in Europe and cryptic speciation within the North Atlantic

Jake Goodall
, Kristen Marie Westfall
, Hildur Magnúsdóttir
, Snæbjörn Pálsson
, Erla Björk Örnólfsdóttir
, Zophonías O. Jónsson

University of Iceland, Holar University College, Other Icelandic Research Institutions

Research output: Contribution to journal › Article › peer-review

Abstract

Buccinum undatum is a subtidal gastropod that exhibits clear spatial variation in several phenotypic shell traits (color, shape, and thickness) across its North Atlantic distribution. Studies of spatial phenotypic variation exist for the species; however, population genetic studies have thus far relied on a limited set of mitochondrial and microsatellite markers. Here, we greatly expand on previous work by characterizing population genetic structure in B. undatum across the North Atlantic from SNP variation obtained by RAD sequencing. There was a high degree of genetic differentiation between Canadian and European populations (Iceland, Faroe Islands, and England) consistent with the divergence of populations in allopatry (F_ST > 0.57 for all pairwise comparisons). In addition, B. undatum populations within Iceland, the Faroe Islands, and England are typified by weak but significant genetic structuring following an isolation-by-distance model. Finally, we established a significant correlation between genetic structuring in Iceland and two phenotypic traits: shell shape and color frequency. The works detailed here enhance our understanding of genetic structuring in B. undatum and establish the species as an intriguing model for future genome-wide association studies.

Original language	English
Pages (from-to)	2616-2629
Number of pages	14
Journal	Ecology and Evolution
Volume	11
Issue number	6
DOIs	https://doi.org/10.1002/ece3.7219
Publication status	Published - 9 Feb 2021

Bibliographical note

Funding Information: The project was funded by research grants #141302‐051 and #196417‐051 from the Icelandic Centre for Research (RANNÍS). We want to thank Símon Sturluson, the captain of Ronja SH‐53, and Will Butler for their assistance in sampling in Breiðafjörður. We are also immensely grateful to Bernard Sainte‐Marie (Maurice Lamontagne Institute, Department of Fisheries and Oceans, Canada), Una Matras (Havstovan in the Faroe Islands), and Kathryn E. Smith (University of Exeter, England) for providing us with samples from their respective regions. We are very grateful to Ólafur Th. Magnússon at deCODE Genetics for advice and the Illumina sequencing. We greatly appreciate valuable input from Hörður Guðmundsson, Kalina Kapralova, Denis Warshan, Charles Hansen, Marcos Lagunas, and Han Xiao, who participated in several discussions on population genetic theory and methodologies during the development of this project. Funding Information: The project was funded by research grants #141302-051 and #196417-051 from the Icelandic Centre for Research (RANN?S). We want to thank S?mon Sturluson, the captain of Ronja SH-53, and Will Butler for their assistance in sampling in Brei?afj?r?ur. We are also immensely grateful to Bernard Sainte-Marie (Maurice Lamontagne Institute, Department of Fisheries and Oceans, Canada), Una Matras (Havstovan in the Faroe Islands), and Kathryn E. Smith (University of Exeter, England) for providing us with samples from their respective regions. We are very grateful to ?lafur Th. Magn?sson at deCODE Genetics for advice and the Illumina sequencing. We greatly appreciate valuable input from H?r?ur Gu?mundsson, Kalina Kapralova, Denis Warshan, Charles Hansen, Marcos Lagunas, and Han Xiao, who participated in several discussions on population genetic theory and methodologies during the development of this project. Publisher Copyright: © 2021 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.

Other keywords

F
divergence
genetics
phylogeography
speciation

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10.1002/ece3.7219

Cite this

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title = "RAD sequencing of common whelk, Buccinum undatum, reveals fine-scale population structuring in Europe and cryptic speciation within the North Atlantic",

abstract = "Buccinum undatum is a subtidal gastropod that exhibits clear spatial variation in several phenotypic shell traits (color, shape, and thickness) across its North Atlantic distribution. Studies of spatial phenotypic variation exist for the species; however, population genetic studies have thus far relied on a limited set of mitochondrial and microsatellite markers. Here, we greatly expand on previous work by characterizing population genetic structure in B. undatum across the North Atlantic from SNP variation obtained by RAD sequencing. There was a high degree of genetic differentiation between Canadian and European populations (Iceland, Faroe Islands, and England) consistent with the divergence of populations in allopatry (FST > 0.57 for all pairwise comparisons). In addition, B. undatum populations within Iceland, the Faroe Islands, and England are typified by weak but significant genetic structuring following an isolation-by-distance model. Finally, we established a significant correlation between genetic structuring in Iceland and two phenotypic traits: shell shape and color frequency. The works detailed here enhance our understanding of genetic structuring in B. undatum and establish the species as an intriguing model for future genome-wide association studies.",

keywords = "F, divergence, genetics, phylogeography, speciation",

author = "Jake Goodall and Westfall, \{Kristen Marie\} and Hildur Magn{\'u}sd{\'o}ttir and Sn{\ae}bj{\"o}rn P{\'a}lsson and {\"O}rn{\'o}lfsd{\'o}ttir, \{Erla Bj{\"o}rk\} and J{\'o}nsson, \{Zophon{\'i}as O.\}",

note = "Funding Information: The project was funded by research grants \#141302‐051 and \#196417‐051 from the Icelandic Centre for Research (RANN{\'I}S). We want to thank S{\'i}mon Sturluson, the captain of Ronja SH‐53, and Will Butler for their assistance in sampling in Brei{\dh}afj{\"o}r{\dh}ur. We are also immensely grateful to Bernard Sainte‐Marie (Maurice Lamontagne Institute, Department of Fisheries and Oceans, Canada), Una Matras (Havstovan in the Faroe Islands), and Kathryn E. Smith (University of Exeter, England) for providing us with samples from their respective regions. We are very grateful to {\'O}lafur Th. Magn{\'u}sson at deCODE Genetics for advice and the Illumina sequencing. We greatly appreciate valuable input from H{\"o}r{\dh}ur Gu{\dh}mundsson, Kalina Kapralova, Denis Warshan, Charles Hansen, Marcos Lagunas, and Han Xiao, who participated in several discussions on population genetic theory and methodologies during the development of this project. Funding Information: The project was funded by research grants \#141302-051 and \#196417-051 from the Icelandic Centre for Research (RANN?S). We want to thank S?mon Sturluson, the captain of Ronja SH-53, and Will Butler for their assistance in sampling in Brei?afj?r?ur. We are also immensely grateful to Bernard Sainte-Marie (Maurice Lamontagne Institute, Department of Fisheries and Oceans, Canada), Una Matras (Havstovan in the Faroe Islands), and Kathryn E. Smith (University of Exeter, England) for providing us with samples from their respective regions. We are very grateful to ?lafur Th. Magn?sson at deCODE Genetics for advice and the Illumina sequencing. We greatly appreciate valuable input from H?r?ur Gu?mundsson, Kalina Kapralova, Denis Warshan, Charles Hansen, Marcos Lagunas, and Han Xiao, who participated in several discussions on population genetic theory and methodologies during the development of this project. Publisher Copyright: {\textcopyright} 2021 The Authors. Ecology and Evolution published by John Wiley \& Sons Ltd.",

year = "2021",

month = feb,

day = "9",

doi = "10.1002/ece3.7219",

language = "English",

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journal = "Ecology and Evolution",

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AU - Goodall, Jake

AU - Westfall, Kristen Marie

AU - Magnúsdóttir, Hildur

AU - Pálsson, Snæbjörn

AU - Örnólfsdóttir, Erla Björk

AU - Jónsson, Zophonías O.

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PY - 2021/2/9

Y1 - 2021/2/9

N2 - Buccinum undatum is a subtidal gastropod that exhibits clear spatial variation in several phenotypic shell traits (color, shape, and thickness) across its North Atlantic distribution. Studies of spatial phenotypic variation exist for the species; however, population genetic studies have thus far relied on a limited set of mitochondrial and microsatellite markers. Here, we greatly expand on previous work by characterizing population genetic structure in B. undatum across the North Atlantic from SNP variation obtained by RAD sequencing. There was a high degree of genetic differentiation between Canadian and European populations (Iceland, Faroe Islands, and England) consistent with the divergence of populations in allopatry (FST > 0.57 for all pairwise comparisons). In addition, B. undatum populations within Iceland, the Faroe Islands, and England are typified by weak but significant genetic structuring following an isolation-by-distance model. Finally, we established a significant correlation between genetic structuring in Iceland and two phenotypic traits: shell shape and color frequency. The works detailed here enhance our understanding of genetic structuring in B. undatum and establish the species as an intriguing model for future genome-wide association studies.

AB - Buccinum undatum is a subtidal gastropod that exhibits clear spatial variation in several phenotypic shell traits (color, shape, and thickness) across its North Atlantic distribution. Studies of spatial phenotypic variation exist for the species; however, population genetic studies have thus far relied on a limited set of mitochondrial and microsatellite markers. Here, we greatly expand on previous work by characterizing population genetic structure in B. undatum across the North Atlantic from SNP variation obtained by RAD sequencing. There was a high degree of genetic differentiation between Canadian and European populations (Iceland, Faroe Islands, and England) consistent with the divergence of populations in allopatry (FST > 0.57 for all pairwise comparisons). In addition, B. undatum populations within Iceland, the Faroe Islands, and England are typified by weak but significant genetic structuring following an isolation-by-distance model. Finally, we established a significant correlation between genetic structuring in Iceland and two phenotypic traits: shell shape and color frequency. The works detailed here enhance our understanding of genetic structuring in B. undatum and establish the species as an intriguing model for future genome-wide association studies.

KW - F

KW - divergence

KW - genetics

KW - phylogeography

KW - speciation

UR - https://www.scopus.com/pages/publications/85100774022

U2 - 10.1002/ece3.7219

DO - 10.1002/ece3.7219

M3 - Article

SN - 2045-7758

VL - 11

SP - 2616

EP - 2629

JO - Ecology and Evolution

JF - Ecology and Evolution

IS - 6

ER -

RAD sequencing of common whelk, Buccinum undatum, reveals fine-scale population structuring in Europe and cryptic speciation within the North Atlantic

Abstract

Bibliographical note

Other keywords

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