Abstract
Denaturing HPLC was used to determine mutations occurring during the adaptive evolution of Escherichia coli K-12. The strains were evolved over 700 generations on glycerol as the sole carbon source from a sub-optimal to an optimal growth rate. The mutations detected by direct sequencing of amplicons of the glycerol-phosphate regulon repressor (glpR) gene were a synonymous substitution Val20Val in two separately evolved strains. Nonsynonymous substitutions, Val119Gly and Gly179Trp, were also observed in each of the two strains. This procedure can be scaled to determine genome-scale sequence variations that have occurred during adaptive evolution.
| Original language | English |
|---|---|
| Pages (from-to) | 435-441 |
| Number of pages | 7 |
| Journal | Biotechnology Letters |
| Volume | 25 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Mar 2003 |
Other keywords
- Adaptive evolution
- Denaturing HPLC
- Escherichia coli
- Mutation sequence variation
- Phenotype
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