Some properties of the ribosomal RNA methyltransferase encoded by ksgA and the polarity of ksgA transcription

Ólafur Sigmar Andrésson; Julian E. Davies

doi:10.1007/BF00268466

Some properties of the ribosomal RNA methyltransferase encoded by ksgA and the polarity of ksgA transcription

Ólafur Sigmar Andrésson, Julian E. Davies

Faculty of Life and Environmental Sciences

University of Iceland

Research output: Contribution to journal › Article › peer-review

Abstract

The assay for the ksgA-encoded S-adenosylmethionine-- 6-N′, N′-adenosyl (rRNA) dimethyltransferase has been improved; the gel-filtration molecular weight of partially purified enzyme under two different sets of conditions was found to be 55,000 or 26,000 daltons. We have determined methyltransferase activities in strains where ksgA was brought under the control of the mitomycin C-inducible promoter of the colicin El gene. Our studies show that ksgA is transcribed counterclockwise on the Escherichia coli chromosome.

Original language	English
Pages (from-to)	217-222
Number of pages	6
Journal	MGG Molecular & General Genetics
Volume	179
Issue number	1
DOIs	https://doi.org/10.1007/BF00268466
Publication status	Published - Aug 1980

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abstract = "The assay for the ksgA-encoded S-adenosylmethionine-- 6-N′, N′-adenosyl (rRNA) dimethyltransferase has been improved; the gel-filtration molecular weight of partially purified enzyme under two different sets of conditions was found to be 55,000 or 26,000 daltons. We have determined methyltransferase activities in strains where ksgA was brought under the control of the mitomycin C-inducible promoter of the colicin El gene. Our studies show that ksgA is transcribed counterclockwise on the Escherichia coli chromosome.",

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Some properties of the ribosomal RNA methyltransferase encoded by ksgA and the polarity of ksgA transcription

Abstract

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