TY - JOUR
T1 - The crest phenotype in chicken is associated with ectopic expression of hoxc8 in cranial skin
AU - Wang, Yanqiang
AU - Gao, Yu
AU - Imsland, Freyja
AU - Gu, Xiaorong
AU - Feng, Chungang
AU - Liu, Ranran
AU - Song, Chi
AU - Tixier-Boichard, Michèle
AU - Gourichon, David
AU - Li, Qingyuan
AU - Chen, Kuanwei
AU - Li, Huifang
AU - Andersson, Leif
AU - Hu, Xiaoxiang
AU - Li, Ning
PY - 2012/4/13
Y1 - 2012/4/13
N2 - The Crest phenotype is characterised by a tuft of elongated feathers atop the head. A similar phenotype is also seen in several wild bird species. Crest shows an autosomal incompletely dominant mode of inheritance and is associated with cerebral hernia. Here we show, using linkage analysis and genome-wide association, that Crest is located on the E22C19W28 linkage group and that it shows complete association to the HOXC-cluster on this chromosome. Expression analysis of tissues from Crested and non-crested chickens, representing 26 different breeds, revealed that HOXC8, but not HOXC12 or HOXC13, showed ectopic expression in cranial skin during embryonic development. We propose that Crest is caused by a cis-acting regulatory mutation underlying the ectopic expression of HOXC8. However, the identification of the causative mutation(s) has to await until a method becomes available for assembling this chromosomal region. Crest is unfortunately located in a genomic region that has so far defied all attempts to establish a contiguous sequence.
AB - The Crest phenotype is characterised by a tuft of elongated feathers atop the head. A similar phenotype is also seen in several wild bird species. Crest shows an autosomal incompletely dominant mode of inheritance and is associated with cerebral hernia. Here we show, using linkage analysis and genome-wide association, that Crest is located on the E22C19W28 linkage group and that it shows complete association to the HOXC-cluster on this chromosome. Expression analysis of tissues from Crested and non-crested chickens, representing 26 different breeds, revealed that HOXC8, but not HOXC12 or HOXC13, showed ectopic expression in cranial skin during embryonic development. We propose that Crest is caused by a cis-acting regulatory mutation underlying the ectopic expression of HOXC8. However, the identification of the causative mutation(s) has to await until a method becomes available for assembling this chromosomal region. Crest is unfortunately located in a genomic region that has so far defied all attempts to establish a contiguous sequence.
UR - https://www.scopus.com/pages/publications/84859713572
U2 - 10.1371/journal.pone.0034012
DO - 10.1371/journal.pone.0034012
M3 - Article
C2 - 22514613
SN - 1932-6203
VL - 7
JO - PLoS ONE
JF - PLoS ONE
IS - 4
M1 - e34012
ER -