The ontogenic transcription of complement component C3 and Apolipoprotein A-I tRNA in Atlantic cod (Gadus morhua L.) - A role in development and homeostasis?

Sigrun Lange; Alister W. Dodds; Sigrídur Gudmundsdóttir; Slavko H. Bambir; Bergljót Magnadóttir

doi:10.1016/j.dci.2005.03.009

The ontogenic transcription of complement component C3 and Apolipoprotein A-I tRNA in Atlantic cod (Gadus morhua L.) - A role in development and homeostasis?

Sigrun Lange
, Alister W. Dodds
, Sigrídur Gudmundsdóttir
, Slavko H. Bambir
, Bergljót Magnadóttir

Faculty of Health Promotion, Sports and Leisure Studies

University of Iceland

Research output: Contribution to journal › Article › peer-review

Abstract

The complement system is important both in the innate and adaptive immune response, with C3 as the central protein of all three activation pathways. Apolipoprotein A-I (ApoLP A-I), a high-density lipoprotein (HDL), has been shown to have a regulatory role in the complement system by inhibiting the formation of the membrane attack complex (MAC). Complement has been associated with apoptotic functions, which are important in the immune response and are involved in organ formation and homeostasis. mRNA probes for cod C3 and ApoLP A-I were synthesized and in situ hybridisation used to monitor the ontogenic development of cod from fertilised eggs until 57 days after hatching. Both C3 and ApoLP A-I transcription was detected in the central nervous system (CNS), eye, kidney, liver, muscle, intestines, skin and chondrocytes at different stages of development. Using TUNEL staining, apoptotic cells were identified within the same areas from 4 to 57 days posthatching. The present findings may suggest a role for C3 and ApoLP A-I during larval development and a possible role in the homeostasis of various organs in cod.

Original language	English
Pages (from-to)	1065-1077
Number of pages	13
Journal	Developmental and Comparative Immunology
Volume	29
Issue number	12
DOIs	https://doi.org/10.1016/j.dci.2005.03.009
Publication status	Published - 2005

Bibliographical note

Funding Information: The authors wish to thank Agnar Steinarsson, Matthías Oddgeirsson and the staff at Stadur, Grindavík, Iceland, for providing the fish and sampling facilities. Thanks are also due to Margrét Jónsdóttir at Keldur, Institute for Experimental Pathology University of Iceland, for preparation of cod larvae samples and tissue sections. Thanks to Professor Jürg A. Schifferli, University Hospital Basel, Switzerland, for providing research facilities for part of this work, and to Brigitte Schneider and Kwok Min Hui, University Hospital Basel, for technical assistance. This work was supported by the EC grant FISHAID QLRT-1999-31076, The Icelandic Ministry for Fisheries, The Icelandic Research Council, The European Moleacular Biology Organisation (EMBO), The Nordic Organisation for Fish Immunology (NOFFI), The International Union of Biochemistry and Molecular Biology (IUBMB) and the Swiss National Science Foundation.

Other keywords

Apolipoprotein A-I
Apoptosis
C3
Cod (Gadus morhua L.)
Complement
Development
Embryo
Ontogeny

Access to Document

10.1016/j.dci.2005.03.009

Cite this

@article{34b8527d81a74970b5a38e12a09e5b70,

title = "The ontogenic transcription of complement component C3 and Apolipoprotein A-I tRNA in Atlantic cod (Gadus morhua L.) - A role in development and homeostasis?",

abstract = "The complement system is important both in the innate and adaptive immune response, with C3 as the central protein of all three activation pathways. Apolipoprotein A-I (ApoLP A-I), a high-density lipoprotein (HDL), has been shown to have a regulatory role in the complement system by inhibiting the formation of the membrane attack complex (MAC). Complement has been associated with apoptotic functions, which are important in the immune response and are involved in organ formation and homeostasis. mRNA probes for cod C3 and ApoLP A-I were synthesized and in situ hybridisation used to monitor the ontogenic development of cod from fertilised eggs until 57 days after hatching. Both C3 and ApoLP A-I transcription was detected in the central nervous system (CNS), eye, kidney, liver, muscle, intestines, skin and chondrocytes at different stages of development. Using TUNEL staining, apoptotic cells were identified within the same areas from 4 to 57 days posthatching. The present findings may suggest a role for C3 and ApoLP A-I during larval development and a possible role in the homeostasis of various organs in cod.",

keywords = "Apolipoprotein A-I, Apoptosis, C3, Cod (Gadus morhua L.), Complement, Development, Embryo, Ontogeny",

author = "Sigrun Lange and Dodds, \{Alister W.\} and Sigr{\'i}dur Gudmundsd{\'o}ttir and Bambir, \{Slavko H.\} and Berglj{\'o}t Magnad{\'o}ttir",

note = "Funding Information: The authors wish to thank Agnar Steinarsson, Matth{\'i}as Oddgeirsson and the staff at Stadur, Grindav{\'i}k, Iceland, for providing the fish and sampling facilities. Thanks are also due to Margr{\'e}t J{\'o}nsd{\'o}ttir at Keldur, Institute for Experimental Pathology University of Iceland, for preparation of cod larvae samples and tissue sections. Thanks to Professor J{\"u}rg A. Schifferli, University Hospital Basel, Switzerland, for providing research facilities for part of this work, and to Brigitte Schneider and Kwok Min Hui, University Hospital Basel, for technical assistance. This work was supported by the EC grant FISHAID QLRT-1999-31076, The Icelandic Ministry for Fisheries, The Icelandic Research Council, The European Moleacular Biology Organisation (EMBO), The Nordic Organisation for Fish Immunology (NOFFI), The International Union of Biochemistry and Molecular Biology (IUBMB) and the Swiss National Science Foundation.",

year = "2005",

doi = "10.1016/j.dci.2005.03.009",

language = "English",

volume = "29",

pages = "1065--1077",

journal = "Developmental and Comparative Immunology",

issn = "0145-305X",

publisher = "Elsevier Ltd.",

number = "12",