Construction and characterization of an infectious molecular clone of maedi-visna virus that expresses green fluorescent protein

Hólmfrídur Sunna Gudmundsdóttir; Katrín Olafsdóttir; Sigrídur Rut Franzdóttir; Valgerdur Andrésdóttir

doi:10.1016/j.jviromet.2010.04.026

Construction and characterization of an infectious molecular clone of maedi-visna virus that expresses green fluorescent protein

Hólmfrídur Sunna Gudmundsdóttir
, Katrín Olafsdóttir
, Sigrídur Rut Franzdóttir
, Valgerdur Andrésdóttir

Líf- og umhverfisvísindadeild

Háskóli Íslands

Rannsóknarafurð: Framlag til fræðitímarits › Grein › ritrýni

Útdráttur

The construction of a molecular clone of maedi-visna virus (MVV) expressing the enhanced green fluorescent protein (EGFP) is described. The egfp gene was inserted into the gene for dUTPase since it has been shown that dUTPase is dispensable for MVV replication both in vitro and in vivo. MVV-egfp is infectious and EGFP expression is stable over at least six passages. This fluorescent virus will be a useful tool for monitoring MVV infections.

Upprunalegt tungumál	Enska
Síður (frá-til)	98-102
Síðufjöldi	5
Fræðitímarit	Journal of Virological Methods
Bindi	168
Númer tölublaðs	1-2
DOI	https://doi.org/10.1016/j.jviromet.2010.04.026
Útgáfustaða	Útgefið - sep. 2010

Athugasemd

Funding Information: This work was supported by grants from The Icelandic Research Fund and the University of Iceland Research Fund .

Aðgangur að skjali

10.1016/j.jviromet.2010.04.026

Vitna í þetta

@article{2dc33559981b4408a9a783660efe4bd4,

title = "Construction and characterization of an infectious molecular clone of maedi-visna virus that expresses green fluorescent protein",

abstract = "The construction of a molecular clone of maedi-visna virus (MVV) expressing the enhanced green fluorescent protein (EGFP) is described. The egfp gene was inserted into the gene for dUTPase since it has been shown that dUTPase is dispensable for MVV replication both in vitro and in vivo. MVV-egfp is infectious and EGFP expression is stable over at least six passages. This fluorescent virus will be a useful tool for monitoring MVV infections.",

keywords = "EGFP, Lentivirus, Maedi-visna virus (MVV)",

author = "Gudmundsd{\'o}ttir, \{H{\'o}lmfr{\'i}dur Sunna\} and Katr{\'i}n Olafsd{\'o}ttir and Franzd{\'o}ttir, \{Sigr{\'i}dur Rut\} and Valgerdur Andr{\'e}sd{\'o}ttir",

note = "Funding Information: This work was supported by grants from The Icelandic Research Fund and the University of Iceland Research Fund .",

year = "2010",

month = sep,

doi = "10.1016/j.jviromet.2010.04.026",

language = "English",

volume = "168",

pages = "98--102",

journal = "Journal of Virological Methods",

issn = "0166-0934",

publisher = "Elsevier B.V.",

number = "1-2",

}

Construction and characterization of an infectious molecular clone of maedi-visna virus that expresses green fluorescent protein. / Gudmundsdóttir, Hólmfrídur Sunna; Olafsdóttir, Katrín ; Franzdóttir, Sigrídur Rut et al.
Í: Journal of Virological Methods, Bindi 168, Nr. 1-2, 09.2010, s. 98-102.

Rannsóknarafurð: Framlag til fræðitímarits › Grein › ritrýni

TY - JOUR

T1 - Construction and characterization of an infectious molecular clone of maedi-visna virus that expresses green fluorescent protein

AU - Gudmundsdóttir, Hólmfrídur Sunna

AU - Olafsdóttir, Katrín

AU - Franzdóttir, Sigrídur Rut

AU - Andrésdóttir, Valgerdur

N1 - Funding Information: This work was supported by grants from The Icelandic Research Fund and the University of Iceland Research Fund .

PY - 2010/9

Y1 - 2010/9

N2 - The construction of a molecular clone of maedi-visna virus (MVV) expressing the enhanced green fluorescent protein (EGFP) is described. The egfp gene was inserted into the gene for dUTPase since it has been shown that dUTPase is dispensable for MVV replication both in vitro and in vivo. MVV-egfp is infectious and EGFP expression is stable over at least six passages. This fluorescent virus will be a useful tool for monitoring MVV infections.

AB - The construction of a molecular clone of maedi-visna virus (MVV) expressing the enhanced green fluorescent protein (EGFP) is described. The egfp gene was inserted into the gene for dUTPase since it has been shown that dUTPase is dispensable for MVV replication both in vitro and in vivo. MVV-egfp is infectious and EGFP expression is stable over at least six passages. This fluorescent virus will be a useful tool for monitoring MVV infections.

KW - EGFP

KW - Lentivirus

KW - Maedi-visna virus (MVV)

UR - https://www.scopus.com/pages/publications/77955094711

U2 - 10.1016/j.jviromet.2010.04.026

DO - 10.1016/j.jviromet.2010.04.026

M3 - Article

C2 - 20447418

SN - 0166-0934

VL - 168

SP - 98

EP - 102

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 1-2

ER -

Construction and characterization of an infectious molecular clone of maedi-visna virus that expresses green fluorescent protein

Útdráttur

Athugasemd

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