TY - JOUR
T1 - In vitro biological response of human osteoblasts in 3D chitosan sponges with controlled degree of deacetylation and molecular weight
AU - Sukul, Mousumi
AU - Sahariah, Priyanka
AU - Lauzon, Hélène L.
AU - Borges, João
AU - Másson, Már
AU - Mano, João F.
AU - Haugen, Håvard J.
AU - Reseland, Janne E.
N1 - Funding Information: This work was supported by The Marine Biotechnology ERA-NET project, “Blueteeth” (ERA-MBT/0002/2015) financed under EU FP7 (Grant Agreement number: 604814). This is a co-funded project coordinated by the University of Aveiro, Portugal (supported by the Portuguese Foundation for Science and Technology (FCT)), and having the company Primex ehf and University of Iceland, Iceland (supported by the Icelandic Technical Development Fund grant No. 179012-0612) and the University of Oslo, Norway (supported by the Norway Research Council, Project ID 269522/O70) as partners. This work was developed within the scope of the project CICECO – Aveiro Institute of Materials, UIDB/50011/2020 & UIDP/50011/2020, financed by national funds through the Foundation for Science and Technology/MCTES . J. Borges gratefully acknowledges the financial support by FCT through his individual contract (CEECIND/03202/2017). The authors would like to thank Aina-Mari Lian for the contribution in Luminex experiment and Liebert Parreiras Nogueira for nano CT analyses and, and Dr. Svetlana Solodova for assistance with the GPC analysis. Publisher Copyright: © 2020 The Author(s)
PY - 2021/2/1
Y1 - 2021/2/1
N2 - We have studied the effect of chitosan sponges, produced from chitosan batches with distinct degree of deacetylation (DDA) and molecular weight (Mw), on the adhesion, growth and differentiation of primary human osteoblasts with an aim to offer a suitable tool for guided bone regeneration. All the chitosan sponges revealed similar microstructure, irrespective of the DDA (58, 73, 82, 88, and 91 %) and Mw (749, 547, 263, 215, and 170 kDa, respectively). Cell spreading was higher on sponges having a higher DDA. Higher DDA induced a more pronounced increase in alkaline phosphatase activity, osteopontin (OPN), vascular endothelial growth factor-A (VEGF), interleukin-6 (IL-6), and reduction in monocyte chemoattractant protein-1 (MCP-1), sclerostin (SOST) and dickkopf related protein-1 as compared to lower DDA. Lower DDA induced the increased secretion of osteoprotegerin and SOST as compared to higher DDA. The combination of higher DDA and Mw induced an increased secretion of VEGF and IL-6, however reduced the secretion of OPN as compared to chitosan with similar DDA but with lower Mw. In summary, the variations in cellular responses to the different chitosan sponges indicate a potential for individual tailoring of desired responses in guided bone regeneration.
AB - We have studied the effect of chitosan sponges, produced from chitosan batches with distinct degree of deacetylation (DDA) and molecular weight (Mw), on the adhesion, growth and differentiation of primary human osteoblasts with an aim to offer a suitable tool for guided bone regeneration. All the chitosan sponges revealed similar microstructure, irrespective of the DDA (58, 73, 82, 88, and 91 %) and Mw (749, 547, 263, 215, and 170 kDa, respectively). Cell spreading was higher on sponges having a higher DDA. Higher DDA induced a more pronounced increase in alkaline phosphatase activity, osteopontin (OPN), vascular endothelial growth factor-A (VEGF), interleukin-6 (IL-6), and reduction in monocyte chemoattractant protein-1 (MCP-1), sclerostin (SOST) and dickkopf related protein-1 as compared to lower DDA. Lower DDA induced the increased secretion of osteoprotegerin and SOST as compared to higher DDA. The combination of higher DDA and Mw induced an increased secretion of VEGF and IL-6, however reduced the secretion of OPN as compared to chitosan with similar DDA but with lower Mw. In summary, the variations in cellular responses to the different chitosan sponges indicate a potential for individual tailoring of desired responses in guided bone regeneration.
KW - Acetylation
KW - Adaptor Proteins, Signal Transducing/genetics
KW - Alkaline Phosphatase/genetics
KW - Biomarkers/metabolism
KW - Bone Regeneration
KW - Cell Proliferation/drug effects
KW - Chemokine CCL2/genetics
KW - Chitosan/chemistry
KW - Delayed-Action Preparations/chemistry
KW - Gene Expression/drug effects
KW - Humans
KW - Intercellular Signaling Peptides and Proteins/genetics
KW - Interleukin-6/genetics
KW - Models, Biological
KW - Molecular Structure
KW - Molecular Weight
KW - Osteoblasts/cytology
KW - Osteopontin/genetics
KW - Osteoprotegerin/genetics
KW - Primary Cell Culture
KW - Vascular Endothelial Growth Factor A/genetics
UR - https://www.scopus.com/pages/publications/85097635748
U2 - 10.1016/j.carbpol.2020.117434
DO - 10.1016/j.carbpol.2020.117434
M3 - Article
C2 - 33357907
SN - 0144-8617
VL - 254
SP - 117434
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
M1 - 117434
ER -